Ayyukan rigakafin ƙwayoyin cuta

Ayyukan rigakafin ƙwayoyin cuta

1. Slant inoculation (tare da Staphylococcus aureus)
(1) Kafin a yi aiki, shafa hannuwanku da barasa 75%, kuma kunna fitilar barasa bayan barasa ya ƙafe.
(2) Rike bututun damuwa da jirgin da ke karkata tsakanin yatsan hannun hagu da sauran yatsu guda hudu, ta yadda jirgin da ke karkata da gefen da ke da nau'i ya kasance sama da a kwance.
(3) A fara jujjuya nau'in da tampon a kan jirgin da aka karkata, ta yadda za a iya fitar da shi cikin sauki yayin allurar.
(4) Rike zoben inoculation a hannun hagu (kamar riƙe alƙalami), sannan a basar ƙarshen zoben da harshen wuta, sa'an nan kuma sanya sauran bututun gwajin da zai iya shiga cikin bututun gwajin.
(5) A yi amfani da yatsan zobe, ɗan yatsa da tafin hannun dama don zazzage bututun datti da filogin auduga ko hular bututun gwajin da ake son haɗawa a lokaci guda, sannan a bar bakin bututun gwajin. a kasance mai zafi a hankali don bakara (kada ku ƙone shi da zafi sosai).
(6) Sai a mika madaukin da ya kone a cikin bututun al'adar bakteriya, a taba mad'in allurar da ke jikin bangon ciki na bututun gwajin ko matsakaicin ba tare da gasasshen kwayoyin cuta ba, sai a bar shi ya huce, sannan a rika goge guntun kwayoyin cuta kadan, sannan a cire. kwayoyin cuta daga kwayoyin cuta.Cire madaukin inoculation daga bututun iri.
(7).Daga kasa na bevel zuwa sama, yi layi mai yawa a cikin siffar "Z" baya da gaba.Wani lokaci kuma ana iya amfani da allurar rigakafin don zana layi a tsakiyar tsakiyar tsaka-tsaki don yin allurar da ba ta dace ba, don lura da halayen girma na iri..
(7).Daga kasa na bevel zuwa sama, yi layi mai yawa a cikin siffar "Z" baya da gaba.Wani lokaci kuma ana iya amfani da allurar rigakafin don zana layi a tsakiyar tsakiyar tsaka-tsaki don yin allurar da ba ta dace ba, don lura da halayen girma na iri.
(8) Bayan an gama allurar sai a fitar da zoben rigakafin don kona bakin bututun a toshe shi da auduga.
(9) Bakara zoben allurar ta hanyar kona shi da ja.Ajiye madaukin inoculating kuma ƙara filogin auduga.
2. Liquid inoculation
(1) Matsakaicin slanted an haɗa shi da matsakaicin ruwa.Ana amfani da wannan hanyar don lura da halayen girma na ƙwayoyin cuta da ƙaddarar halayen kwayoyin halitta.Hanyar aiki iri ɗaya ce kamar da, amma bakin bututun gwajin yana karkata zuwa sama don hana ruwan al'ada fita bayan an shigar da ƙwayoyin cuta., Shafa zoben inoculation da bangon ciki na bututun wasu lokuta don wanke ƙwayoyin cuta akan ƙananan zobe.Bayan allurar, toshe filogin auduga sannan a matsa bututun gwajin a hankali a tafin hannun don tarwatsa kwayoyin cutar gaba daya.
(2) Sanya matsakaicin ruwa daga matsakaicin ruwa.Lokacin da nau'in ya zama ruwa, yi amfani da pipette mara kyau ko dropper don haɗin gwiwa ban da madaukin inoculation.Lokacin yin alluran, kawai cire filogin auduga kusa da harshen wuta, wuce bututun ƙarfe ta cikin harshen wuta, tsotsa ruwan kwayan cuta a cikin maganin al'ada tare da bakararre pipette, sannan a girgiza sosai.
3. Alurar riga kafi
Bakteriya sun yi tsiri kuma sun bazu akan faranti.
(1) Yin allura ta hanyar ɗigo Dubi hanyar rabuwa.
(2) Rufewa da yin allura bayan tsotsa maganin ƙwayar cuta a cikin farantin tare da bakararre pipette, a watsa shi a ko'ina a saman farantin tare da sandar gilashin da ba ta dace ba.
4. Maganin huda
Ana shigar da nau'ikan cikin matsakaici mai zurfi mai zurfi.Ana amfani da wannan hanyar don rigakafin ƙwayoyin cuta na anaerobic ko don lura da kaddarorin ilimin lissafi lokacin gano ƙwayoyin cuta.
(1) Hanyar aiki iri ɗaya ce da na sama, amma allurar rigakafin da ake amfani da ita yakamata ta kasance madaidaiciya.
(2) A huda allurar rigakafin daga tsakiyar cibiyar al'ada har sai ta kusa da kasan bututun, amma kar a kutsa cikin ta, sannan a cire ta a hankali ta hanyar hanyar huda ta asali.